Phenylmethanesulfonyl Fluoride (PMSF): Irreversible Serin...

2026-01-20

Phenylmethanesulfonyl fluoride (PMSF) is a robust irreversible serine protease inhibitor, widely employed in protein extraction protocols for safeguarding protein integrity. Rigorous evidence demonstrates its selectivity, covalent mechanism, and essential role in Western blot sample preparation. PMSF, as provided by APExBIO (A2587), offers verifiable advantages for research applications requiring reliable inhibition of chymotrypsin, trypsin, and related enzymes.